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Cloning and Expression
Enzymes
EcoRI
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EcoRI
EcoRI
EcoRI from Promega
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Citations:
(24)
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Promega for
EcoRI
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Description
G/AATT C C TTAA\G
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
EcoRI
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
R6011
Quantity
5,000u
Concentration
12u/µl
Sequence
G▼AATT C
C TTAA▲G
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (24)
(1)
DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation
Nucleic Acids Research
October 13, 2023
Georgia Kingsley, Aggeliki Skagia, Paolo Passaretti, Cyntia Fernandez-Cuesta, Alicja Reynolds-Winczura, Kinga Koscielniak, Agnieszka Gambus
in DMSO at 8 mM and added to the extract along with demembranated
sperm
chromatin
at 40 μM. EcoR1 (
R6011
,
Promega
) was purchased at stock 12 U/μl and added to the extract at 0.05 U/μl. Mitomycin C (Calbiochem)
(2)
TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells
Nature communications
August 21, 2023
Scaramuzza S, Jones RM, Sadurni MM, Reynolds-Winczura A, Poovathumkadavil D, Farrell A, Natsume T, Rojas P, Cuesta CF, Kanemaki MT,
et al
.
Committee and by UK home office project license no: P081C27D8.
Inhibitors
and recombinant proteins EcoR1 (
R6011
,
Promega
) was purchased at stock 12 U/µl and added to the extract at 0.05 U/µl,
Aphidicolin
(
A0781
,
(3)
Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1
Journal of Chemical Information and Modeling
April 10, 2023
Alessandro Nicoli, Franziska Haag, Patrick Marcinek, Ruiming He, Johanna Kreißl, Jörg Stein, Alessandro Marchetto, Andreas Dunkel, Thomas Hofmann, Dietmar Krautwurst,
et al.
into expression vectors was done with
T4-DNA ligase
(#
M1804
,
Promega
, Madison, USA) via
EcoRI
/
NotI
(#
R6017
/#
R6435
,
Promega
, Madison, USA) into the expression plasmid pFN210A100 and verified by
Sanger sequencing
(4)
The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster
iScience
November 18, 2022
Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik Hedenström, Björn Bohman, Marie Bengtsson, Paul G. Becher,
et al.
Madison, USA) either
MfeI
/
NotI
(#
R3589S
/ #
R0189S
,
New England Biolabs
, Ipswich, UK) or
EcoRI
/
NotI
(#
R6017
/ #
R6435
,
Promega
, Madison, USA) into the expression plasmid (#pFN210A SS-HaloTag® CMV-neo Flexi®-Vector,
(5)
The p97 segregase cofactor Ubxn7 facilitates replisome disassembly during S-phase
The Journal of biological chemistry
August 1, 2022
Tarcan Z, Poovathumkadavil D, Skagia A, Gambus A.
dissolved in DMSO at 8 mM and added to the extract along with demembranated
sperm
nuclei at 40 μM. EcoR1 (
R6011
,
Promega
) was purchased at stock 12 U/μl and added to the extract at 0.05 U/μl. Recombinant proteins Recombinant
(6)
Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathway
Journal for ImmunoTherapy of Cancer
May 16, 2022
Yue Zhuo et al.
post LLC inoculation by i.p. every 4 days.
RNA pull-down
assays
Restriction endonucleases
EcoRI
(
R6011
,
Promega
) and
SalI
(
R6051
,
Promega
) were used to linearized the
pGEM-3Z vector
(
P2151
,
Promega
), then
(7)
Copper-mediated thiol potentiation and mutagenesis-guided modeling suggest a highly conserved copper-binding motif in human OR2M3
Cellular and Molecular Life Sciences
June 1, 2020
Franziska Haag, Lucky Ahmed, Krystle Reiss, Eric Block, Victor S. Batista, Dietmar Krautwurst
for OR2M3 and OR1A1 (#
R0589S
/#
R0189S
,
New England BioLabs
, Ipswich, USA) or
EcoRI
/
NotI
for OR2W1 (#
R6017
/#
R6435
,
Promega
, Madison, USA) into the expression plasmid pI2-dk(39aa-rho-tag) (aa, amino acid) [53,
(8)
An intestinal zinc sensor regulates food intake and developmental growth
Nature
April 1, 2020
Redhai S, Pilgrim C, Gaspar P, Giesen LV, Lopes T, Riabinina O, Grenier T, Milona A, Chanana B, Swadling JB,
et al
.
Hodor F and Hodor R (see below). The
PCR product
was digested with
NotI
and
EcoRI
(
Promega
#
R6435
and #
R6017
respectively) and cloned into the pUASTattB vector53. hodor-containing pUASTattB was amplified, purified
(9)
Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR
PLOS ONE
June 14, 2018
Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekinge
Moreover, 8.65 μl of untreated DNA samples from all donors was restricted with
EcoRI
enzyme (
Promega
,
R6011
) according to the manufacturer’s instructions. 2μl of this restricted DNA was also used for quantification
(10)
IL-6-HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptors
Journal of Biological Methods
November 3, 2017
Franziska Noe, Tim Frey, Julia Fiedler, Christiane Geithe, Bettina Nowak, Dietmar Krautwurst
products were purified (gel extraction kit, #
28706
,
Qiagen
, Hilden, Germany), digested either
EcoRI
/
NotI
(#
R6017
/ #
R6435
,
Promega
, Madison, USA) or
MfeI
/
NotI
(#
R0589S
/ #
R0189S
,
New England Biolabs
, Ipswich, USA) and
(11)
The genetic audiogenic seizure hamster from Salamanca: The GASH:Sal
Epilepsy & Behavior
June 1, 2017
Luis J. Muñoz, Melissa M. Carballosa-Gautam, Kira Yanowsky, Natividad García-Atarés, Dolores E. López
following: 1× REact I buffer (#Y90002, Invitrogen, Carlsbad, CA, USA), EcoRI restriction enzyme (3.6 U) (#
R6011
,
Promega
, Madison, WI, USA), and MseI restriction enzyme (4.0 U) (#15494-016, Invitrogen). Following
(12)
Sterol O-Acyltransferase 2 Contributes to the Yolk Cholesterol Trafficking during Zebrafish Embryogenesis
PLOS ONE
December 9, 2016
Nai-Yun Chang, Yen-Ju Chan, Shih-Torng Ding, Yen-Hua Lee, Wei-Chun HuangFu, I-Hsuan Liu
(13)
Quorum Sensing Controls Swarming Motility of Burkholderia glumae through Regulation of Rhamnolipids
PLOS ONE
June 5, 2015
Arvin Nickzad, François Lépine, Eric Déziel
(14)
Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
BMC Plant Biology
December 1, 2014
Araceli Nora García, Nicolás Daniel Ayub, Ana Romina Fox, María Cristina Gómez, María José Diéguez, Elba María Pagano, Carolina Andrea Berini, Jorge Prometeo Muschietti, Gabriela Soto
expressing MsSN1, plasmid pSJ33-MsSN1 carrying MsSN1 was constructed by
subcloning
the 0.5-kb
EcoRI
(Cat. #
R6011
,
Promega
) fragment from pCR2.1TOPO-MsSN1 into pSJ33 [28], and afterward introduced in
Escherichia coli
(15)
Characterization of Persistent Virus-Like Particles in Two Acetate-Fed Methanogenic Reactors
PLoS ONE
November 22, 2013
I-Chieh Chien, John Scott Meschke, Heidi L. Gough, John F. Ferguson
spectrophotometer (NanoDrop Technologies, Wilmington, DE). Plasmids were linearized by restriction enzyme
EcoRI
(
R6011
,
Promega
Co., Madison, WI). Reaction mixes contained 2 μl DNA templates, 4.5 μl RealMasterMix and individual
(16)
High-Yield Production in Escherichia coli of Fungal Immunomodulatory Protein Isolated from Flammulina velutipes and Its Bioactivity Assay in Vivo
International Journal of Molecular Sciences
January 24, 2013
Xianghui Kong, Jiechi Zhang, Xue Han, Piqi Zhang, Xiaodong Dai, Jianing Liu, Xinxin Zhang, Imshik Lee, Shenkui Liu
PCR product
was gel purified and then ligated into pET30a (+) at the
BamHI
(R602A,
Promega
) and
EcoRI
(
R6011
,
Promega
) sites. The obtained plasmid was identified by
DNA sequencing
and named pET30a-FIP-fve. 3.3.
(17)
Ocorrência de DNA de herpesvírus bovino tipo 4 em gado Holstein argentino de Santiago del Estero, Argentina
Brazilian Journal of Veterinary Research and Animal Science
December 1, 2011
Sandra Elizabeth Pérez, Andrea Elizabeth Verna, María Rosa Leunda, Paula Ariela Favier, María Carolina Ceriani, Pedro Edgardo Morán, Anselmo Carlos Odeón, Eduardo Néstor Esteban
Qiagen
). DNA obtained from 13 PBL samples was digested with the restriction enzyme
EcoRI
(
Promega
, Cat.
R6011
), according to the manufacturer’s instructions. Digestion products were separated by
electrophoresis
(18)
RDH10 is the primary enzyme responsible for the first step of embryonic Vitamin A metabolism and retinoic acid synthesis
Developmental Biology
September 15, 2011
Krysten M. Farjo, Gennadiy Moiseyev, Olga Nikolaeva, Lisa L. Sandell, Paul A. Trainor, Jian-xing Ma
(19)
Mycoplasma Bovis
Outbreak in a Herd of North American Bison (
Bison Bison
)
Journal of Veterinary Diagnostic Investigation
September 3, 2010
Kyathanahalli S. Janardhan, Mike Hays, Neil Dyer, Richard D. Oberst, Brad M. DeBey
New England Biolabs
Inc., Ipswich, MA. i. Catalog no. 1161-1127, Bio-Rad Laboratories, Hercules, CA. j. Catalog no.
R6011
,
Promega
Corp., Madison, WI. k. Catalog no.
R6381
,
Promega
Corp., Madison, WI. l. Catalog no.
R6021
,
(20)
Inactivation of
Burkholderia cepacia
Complex Phage KS9 gp41 Identifies the Phage Repressor and Generates Lytic Virions
Journal of Virology
February 1, 2010
Karlene H. Lynch, Kimberley D. Seed, Paul Stothard, Jonathan J. Dennis
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EcoRI from Promega
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